When ISO speed is fixed, digital camera has at least six correct exposures that produce identical photo images theoretically.
To take clear and sharp photo without tripod and remote trigger, I put my camera on a box, mirror lock up is on, set the self-timer at two seconds.
The six correct exposures I experimented in my room under fluorescent light is below.
Aperture(f/#) Shutter speed (1/# second) ISO 1600
4.5 125(I made a mistake. It should be 4.0.)
Below two files contain EXIF data.